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Influence of chloramphenicol on growth and respiration of soybean ( Glycine max L.) suspension cultures
Author(s) -
KlerkKiebert Yvonne M.,
Kneppers Tarcies J. A.,
Plas Linus H. W.
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb02270.x
Subject(s) - glycine , chloramphenicol , respiration , alternative oxidase , biochemistry , cytochrome c oxidase , biology , chemistry , mitochondrion , botany , antibiotics , amino acid
By addition of chloramphenicol (CAP) to the growth medium of green soybean ( Glycine max L.) cells in batch culture, growth is inhibited and the activity of the cytochrome oxidase decreases to 60% of the value found in control cells. The presence of CAP induces an enhancement of the contribution of the alternative pathway to total respiration. This enlarged contribution results both from a higher capacity of the alternative pathway and from a greater part of this capacity being used. Also in mitochondria isolated from cells treated with CAP, a higher capacity of the alternative pathway has been found, while the part of this capacity which is really used is comparable with the values found in control cells.