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Metabolism of diclofop‐methyl in cell cultures of Avena sativa and Avena fatua
Author(s) -
Dusky J. A.,
Davies D. G.,
Shimabukuro R. H.
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb00714.x
Subject(s) - avena fatua , avena , metabolism , biology , chemistry , biochemistry , botany , weed
The metabolism of the herbicide, diclofop‐methyl (methyl‐2‐[4‐(2′, 4′‐dichlorophenoxy) phenoxy]propanoate), in cell suspension cultures of Avena sativa L. (cv. Garry) and in callus of Avena fatua L. (transferred to liquid) was determined as a function of time (8 h to about 3 weeks) and was compared to previous metabolism data from intact plants. A. fatua metabolized 14 C‐labeled diclofop‐methyl more rapidly than A. sativa, but the metabolites formed were similar if not identical. Within 2 days, approximately 50% of the total 14 C recovered was in A. fatua cells whereas less than 15% was in A. sativa cells. In older cultures of A. fatua, the amounts of 14 C in the cells and in the medium were about 45% each; 10 to 12% was in the non‐extractable cell residue. The 14 C recovered from A. sativa cells increased to a maximum of about 35% at 7 days and then slowly decreased to about 18% by 21 days, whereas the 14 C in the medium of A. sativa decreased to about 60% at 7 days and then increased to over 75% by 21 days. The nonextractable 14 C residue was 5% or less even after 21 days. Major metabolites in methanolic extracts of cells of both A. sativa and A. fatua were diclofop (2‐[4‐(2′, 4′‐dichlorophenoxy)phenoxy] propanoate), diclofop hydroxylated at an undetermined position on the 2,4‐dichlorophenyl ring (ring OH‐diclofop), and conjugates of diclofop and ring‐OH diclofop.