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Light‐dependent rubidium uptake into isolated mesophyll protoplasts from leaves of Pisum sativum
Author(s) -
Leurs C. J.,
Winter H.,
Wiersema P. K.,
Helder R. J.
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb00349.x
Subject(s) - pisum , protoplast , dcmu , rubidium , sativum , biology , biochemistry , biophysics , botany , photosynthesis , chemistry , photosystem ii , potassium , organic chemistry
A method is described for the isolation of large amounts of physiologically active protoplasts from leaves of Pisum sativum L. Rubidium uptake was determined after separation of the intact protoplasts from the loading medium by rapid centrifugation through a phthalate step gradient. In freshly isolated mesophyll protoplasts of Pisum sativum , rubidium uptake was carbonylcyanide‐ p ‐trifluoromethoxyphenylhydrazone reduced by metabolic inhibitors such as 5 μ M , 0.1 mW cyanide, 2 μ M DCMU and 5 m M arsenate and by dark incubation. Reduction of rubidium uptake by inhibition of aerobic respiration or the photosynthetic electron transport system demonstrates that both processes play a role in the energy supply for membrane transport in these protoplasts.