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Chlorophyll‐protein levels and degree of thylakoid stacking in radish chloroplasts from high‐light, low‐light and bentazon‐treated plants
Author(s) -
Lichtenthaler Hartmut K.,
Kuhn Gertrud,
Prenzel Ursula,
Meier Dieter
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb00322.x
Subject(s) - bentazon , thylakoid , chloroplast , raphanus , chlorophyll , photosystem ii , biology , photosynthesis , chlorophyll a , chlorophyll b , botany , biochemistry , weed , gene
The level of 7 chlorophyll‐carotenoid‐proteins was determined in chloroplasts from radish seedlings ( Raphanus sativus L. var. Saxa Treib) grown in high‐light (HL; 90 W m −2 and low‐light (LL; 10 W m −2 ) growth conditions with and without application of the photosystem 2 herbicide bentazon (10 −4 M ) and compared with the degree of thylakoid stacking.1 The photosynthetic apparatus of HL‐chloroplasts contains higher proportions of the photosystem 1 chlorophyll a ‐proteins CPI and CPIa than LL‐chloroplasts or chloroplasts from bentazon‐treated plants. 2 In LL‐chloroplasts and in chloroplasts from bentazon‐treated plants a higher proportion of light‐harvesting chlorophyll a/b ‐proteins (LHCP 1 , LHCP 2 , LHCP 3 , LHCP y ) is found. Bentazon treatment changes the proportion of LHCPs to about the same levels under both HL and LL‐growth conditions. The amounts of free chlorophyll found in bentazon chloroplasts (27–29%) is higher than in the HL or LL‐controls (16–18%). 3 The increase in degree of thylakoid stacking (% proportion of appressed membranes per total chloroplasts membranes) of LL‐chloroplasts as compared to HL‐chloroplasts of 7 to 9% (3rd to 5th day of illumination) is paralleled by a similar increase in the LHCPs of 5% and 8% (3rd and 5th day). The importance of light harvesting chlorophyll a/b ‐proteins as a prerequisite for thylakoid stacking is discussed.