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Autolytic activities of the cell wall in rice coleoptiles. Effects of nojirimycin
Author(s) -
Labrador Emilia,
Nicolas Gregorio
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb00303.x
Subject(s) - cell wall , autolysis (biology) , coleoptile , polysaccharide , glucan , elongation , glucanase , biochemistry , chemistry , monosaccharide , avena , biology , botany , enzyme , ultimate tensile strength , materials science , metallurgy
Oryza sativa L. var. bahia coleoptile cell walls show sufficient autolytic activity for the release into the surrounding medium of amounts up to 60 μg of sugars per mg of dry weight of cell wall. The products released elute in Bio‐gel P.2 as mono‐ and polysaccharides with glucose as the sole component. The polysaccharide component releases tri‐ and tetrasaccharides on treatment with a glucanase specific for β (1–3) (1–4) linkages in the same proportion as that of the mixed glucan of the cell wall. This supports the hypothesis that the polysaccharide component originates from the cell wall glucan and that autolysis is therefore related to the processes of the loss of rigidity of the cell wall. Nojirimycin (a specific glucanase inhibitor and inhibitor of auxin‐induced elongation) decreases autolytic activity of the cell walls, reducing it to 30% of its normal value. Bio‐gel P. 2 elution of the products released in autolysis in the presence of nojirimycin shows that only the monosaccharide fraction was affected.