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Freeze‐preservation of rice cells: A physiological study of freeze‐thawed cells
Author(s) -
Cella Rino,
Colombo Roberta,
Galli Maria Grazia,
Nielsen Erik,
Rollo Franco,
Sala Francesco
Publication year - 1982
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1982.tb00292.x
Subject(s) - oryza sativa , intracellular , respiration , phosphate , microbiology and biotechnology , protoplast , cell , potassium , biology , cell growth , in vitro , biochemistry , cell culture , chemistry , botany , genetics , organic chemistry , gene
Rice ( Oryza sativa L.) cells returning to in vitro culture after preservation at superlow temperature in liquid nitrogen are characterized by a number of physiological alterations. These include: reduction in respiration and glucose uptake, loss of intracellular potassium, decrease in the cellular level of key metabolites (ATP, glucose‐6‐phosphate and pyruvate) and fragility of protoplasts following the action of cell wall‐degrading enzymes. Nevertheless, cell growth resumes after a short lag phase (2–4 days) with an actual 70–100% cell survival, thus indicating that the observed damage is not lethal and can be repaired in a short time.