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Gibberellin enhancement of the enzymic release of Pisum root cell protoplasts
Author(s) -
Landgren Craig R.
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb06053.x
Subject(s) - protoplast , gibberellin , pisum , sativum , pectinase , cellulase , cell wall , biology , botany , enzyme , biochemistry
Cultivar differences have been reported in the protoplast yields from Pisum sativum root cortical explants treated with preparations of commercial cellulase and pectinase. The presence of intracellular starch significantly influenced these protoplast yields. The application of gibberellin before or during the enzymic wall‐degradation increased the protoplast yields from two of the five cultivars tested. For tissues of‘Little Marvel’ pea roots, 10 mg 1 −1 of gibberellin most effectively increased the release of protoplasts when the hormone preceded the enzyme incubation. One mg 1 −1 of gibberellin was most effective at increasing the protoplast release when the tissues were treated with the hormone simultaneously with the wall‐degrading enzymes. Mitotic activity was significantly reduced in protoplasts derived from gibberellin‐treated tissues.