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Enzymes of the tricarboxylic acid cycle and the malate‐aspartate shuttle in the N 2 ‐fixing endophyte of Alnus glutinosa
Author(s) -
Akkermans Antoon D. L.,
HussDanell Kerstin,
Roelofsen Wim
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb04502.x
Subject(s) - biochemistry , endophyte , isocitrate dehydrogenase , biology , citric acid cycle , malate dehydrogenase , fumarase , root nodule , glutamate dehydrogenase , dehydrogenase , botany , enzyme , bacteria , symbiosis , glutamate receptor , genetics , receptor
The occurrence and localization of enzymes involved in energy supply and biosynthesis was studied in root nodules of Alnus glutinosa (L.) Vill. Vesicle clusters of the endophyte, Frankia sp., contain NADP‐dependent isocitrate dehydrogenase, succinate dehydrogenase, fumarase and malate dehydrogenase. The data indicate that both the endophyte and the host are capable of metabolizing carbon compounds via the tricarboxylic acid cycle. Both vesicle clusters of the endophyte and root nodule cells contain glutamate‐oxaloacetate transaminase which can function in a malate‐aspartate shuttle. This might enable transport of reducing equivalents from the host cell cytoplasm to the endophyte.