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Effect of salicylic acid on nitrite reductase and glutamate dehydrogenase activities in maize roots
Author(s) -
Jain Abha,
Srivastava H. S.
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb04501.x
Subject(s) - nitrite reductase , salicylic acid , glutamate dehydrogenase , nitrate reductase , nitrite , chemistry , glutamate synthase , biochemistry , enzyme , enzyme assay , ammonium , incubation , divalent , dehydrogenase , glutamate receptor , nitrate , organic chemistry , receptor
The effects of 0.01 to 5 m M salicyclic acid on the increase in nitrite reductase or glutamate dehydrogenase activities in maize roots by nitrate or ammonium respectively, were examined. Nitrite reductase activity was inhibited by the highest concentration of the acid. The activity of NADH‐glutamate dehydrogenase was stimulated slightly (but consistently) by the lowest concentration and was inhibited by higher concentrations. Total protein content was also inhibited at high concentrations. When the crude enzyme extract was stored at 25°C in light, the glutamate dehydrogenase activity in the control decreased after 4 h of incubation. Low concentrations of the acid had no effect on this decrease but higher concentration accelerated the process. The divalent cations Caz 2+ , Mn 2+ , Mg 2+ and Zn 2+ protected against loss of enzyme activity during storage, both in the absence and presence of the acid. The inhibitory effect of 5 m M salicylic acid on glutamate dehydrogenase activity is apparent due to interference with the activity of the enzyme rather than with its synthesis.