Premium
Tissue culture of Alhagi camelorum – a legume of high regenerative capacity
Author(s) -
Bharal Shashi,
Rashid A.
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb02739.x
Subject(s) - cytokinin , shoot , explant culture , biology , plantlet , auxin , callus , botany , hypocotyl , tissue culture , in vitro , biochemistry , gene
Alhagi camelorum cultures provide a system with a high propensity for plantlet regeneration from root, hypocotyl, stem, and leaf explants. Excluding leaf explants, all explants regenerate to form shoot‐buds on a simple basal medium suggesting a differential morphogenic potential of different parts of the same plant. All parts of the plant including leaves, form shoot‐buds on cytokinin‐containing media which markedly promote shoot‐bud differentiation, alone or in combination with indoleacetic acid or naphthaleneacetic acid. Rooting occurs on media containing indoleacetic acid and naphthaleneacetic acid. 2, 4‐Dichlorophenoxyacetic acid is inhibitory for differentiation and induces callusing. Callus induced on benzylaminopurine and 2,4‐dichlorophenoxyacetic acid differentiates to form shoot‐buds on transfer to cytokinin‐containing medium. Upon transfer to basal medium shoots produce roots. Plants have been transferred to soil.