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Growth, peroxidase activity, and protein content in stem pith and callus tissues from haploid and diploid Nicotiana tabacum plants
Author(s) -
Kraus Jane E.,
Handro Walter,
Dietrich Sonia M. C.
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb02691.x
Subject(s) - ploidy , callus , pith , peroxidase , biology , nicotiana tabacum , kinetin , nicotiana , botany , explant culture , solanaceae , in vitro , biochemistry , enzyme , gene
Pith segments isolated from haploid and diploid Nicotiana tabacum cv. IAC‐70 plants from five different heights in the stem, were cultured in vitro on MS‐62 medium supplied with 0.5 mg/l IAA and 0.02 mg/l kinetin. Pith tissues of haploid plants showed greater growth potential than those of diploids; peroxidase activity was higher in the calluses of diploid tissues whereas protein content was higher in those of haploids. After three subcultures the growth pattern was inversed, as were the results for peroxidase activity and protein content. After the onset of culture, peroxidase activity in haploid explants dropped sharply, reaching values lower than in diploid tissues 40 d later, but after three subcultures these values were higher than those for diploid calluses. The results are discussed from the viewpoint of a possible relationship between greater polyploidization in haploid tissues on the one hand and growth decrease and peroxidase activity increase on the other.