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Phloem differentiation in Coleus pith explants
Author(s) -
Comer Alan E.,
Leonardo Lois
Publication year - 1981
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1981.tb00890.x
Subject(s) - phloem , pith , coleus , sucrose , xylem , botany , biology , explant culture , parenchyma , sieve tube element , biochemistry , in vitro
A simple tissue culture protocol was used to examine phloem differentiation. Sieve tube elements differentiated from Coleus blumei Benth. pith parenchyma when pith blocks were explanted on to an agar medium containing sucrose and indole acetic acid. Growth regulator concentration had surprisingly little influence on the production of phloem cells. Increasing the availability of sucrose, however, resulted in an enhancement of phloem differentiation. Evidently phloem and xylem differentiation began at the same time in this system, and the concentration of sucrose determined the relative production of phloem cells.