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Membrane lipids in heat injury of spinach chloroplasts
Author(s) -
SANTARIUS KURT A.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb08638.x
Subject(s) - spinacia , spinach , thylakoid , membrane , chloroplast , biochemistry , membrane lipids , chemistry , chloroplast membrane , photosystem i , biophysics , biology , gene
Heat treatment of intact leaves and of isolated thylakoid membranes from spinach ( Spinacia oleracea L . cvs. Monatol and Montako) caused inactivation of photochemical processes such as electron transport through photosystem II and photophos‐phorylation. Membrane lipid analysis demonstrated that heat‐induced damage to thylakoids is not caused by chemical alterations in the lipids such as oxidation of unsaturated fatty acids, or release of free fatty acids due to hydrolysis of lipids. Partial extraction of lipids from isolated chloroplast membranes before and after thermal inactivation do not point to drastic changes in the binding relations of the lipids within the membranes. However, it cannot be excluded that during high temperature treatment changes in lipid‐lipid interactions and/or delocalization of specific lipids within the thylakoids might be responsible for the disorganization of the functional integrity of the membranes. Since thermostability of chloroplast membranes is decreased when they are exposed to free unsaturated fatty acids, small amounts of membrane lipids which become hydrolyzed during extended heat treatment may partly contribute to primary heat damage.