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Effect of ortho‐dihydroxyphenols on growth and protein pattern of callus cultures from Prunus avium
Author(s) -
FEUCHT W.,
SCHMID P. P. S.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb04468.x
Subject(s) - callus , prunus , catechin , chlorogenic acid , polyphenol , shoot , botany , biology , tissue culture , murashige and skoog medium , chemistry , horticulture , biochemistry , in vitro , antioxidant
Excised shoot discs from Prunus avium L. were cultivated on a modified Murashige and Skoog medium for 4 weeks under long day conditions. The basal medium was supplemented with IAA and benzylaminopurine. Further addition of 5.7 × 10 −5 resp, 11.4 × 10 −5 M catechin and 4.25 × 10 −5 resp. 8.5 × 10 −5 M chlorogenic acid resulted in a highly significant promotion of callus proliferation. Proteins and enzymes were separated by isoelectric focusing. The supply of both polyphenols resulted in a higher number and intensity of protein bands including esterases as compared to controls.