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Isolation of active ribulose 1,5‐bisphosphate carboxylase from glanded cotton (G. hirsutum)
Author(s) -
QUAYLE THOMAS J.,
KATTERMAN FRANK R.,
JENSEN RICHARD G.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb04455.x
Subject(s) - ribulose 1,5 bisphosphate , enzyme , pyruvate carboxylase , chemistry , sodium , malvaceae , biochemistry , yield (engineering) , biology , botany , organic chemistry , materials science , metallurgy
Ribulose, 1,5‐bisphosphate carboxylase has been isolated from leaves of glanded cotton, ( Gossypium hirsutum L. cv. TM1), by techniques described. A yield exceeding 4.5 mg enzyme per gram leaf tissue has been obtained by utilizing a sodium homogenizing buffer without the addition of any reducing agent. Sodium dodecylsulfate gel electrophoresis of the enzyme shows the fraction to be relatively free of contaminants. Enzymic assays of the isolate give a specific activity of 1.1 μmol CO 2 fixed (min‐mg protein) −1 at 30°C.