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Reassociation of cytoplasmic ribosomal subunits of barley and its virescens mutant to form active hybrids
Author(s) -
KOUNDAL KIRPA R.,
COHEN ABE S.,
ZALIK SAUL
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb04449.x
Subject(s) - ribosome , cytoplasm , biochemistry , polysome , mutant , ribosomal rna , monomer , hordeum vulgare , centrifugation , biology , chemistry , protein subunit , heterologous , microbiology and biotechnology , biophysics , rna , gene , botany , poaceae , organic chemistry , polymer
Polyphenylalamine synthesis by cytoplasmic ribosomes of Gateway barley ( Hordeum vulgare ) and its virescens single gene nuclear mutant was compared. The cytoplasmic 80S ribosomes were isolated from unimbibed embryo material and the ribosomes were dissociated into their component 60S and 40S subunits by centrifugation through sucrose gradients containing high KCl‐to‐MgCl 2 buffer. These separated subunits could be reassociated by resuspension in buffer having about equimolar concentrations of MgCl 2 and KCl. Both homologous and heterologous combinations of the subunits reassociated to give monomeric 80S ribosomes, and the derived monomers as well as various combinations of the individual subunits showed equivalent activity in an in vitro system for poly (U)‐directed polyphenylalanine synthesis.