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Plantlet formation from embryonic tissue of chestnut grown in vitro
Author(s) -
VIEITEZ ANA M.,
VIEITEZ E.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb04438.x
Subject(s) - primordium , shoot , plantlet , axillary bud , auxin , botany , biology , tissue culture , in vitro , horticulture , biochemistry , gene
Development of axillary shoots was induced when isolated embryonic axes of chestnut ( Castanea sativa Mill.) were cultured on a defined medium containing 6‐benzyl‐aminopurine (BAP). The optimal concentrations of BAP were determined for development of axillary shoots from both embryonic axes and subcultured shoots. After shoot multiplication a great number of shoots have been maintained sequentially without significant change in the proliferative rate for one year. Limited rooting has been obtained with excised shoots. Indole‐3‐butyric acid (IBA) at 2 mg/l was used to induce root primordia. After 8 days of treatment the plantlets were transferred to an auxin‐free medium for root development.