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Metabolism of auxin in pine tissues: Indole‐3‐acetic acid conjugation
Author(s) -
RIOV JOSEPH,
GOTTLIEB HUGO E.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb04112.x
Subject(s) - metabolite , thin layer chromatography , chemistry , chromatography , bark (sound) , indole test , acetic acid , indole 3 acetic acid , column chromatography , auxin , biochemistry , biology , ecology , gene
Incubation of sections of various tissues of Pinus pinea L. with a relatively low concentration (3.6 μ M ) of indole‐3‐acetic acid‐2‐ 14 C (IAA) resulted in the formation of two major metabolites. The first, which has not been identified, seemed to be a polar acidic compound and the second was identified as indole‐3‐acetylaspartic acid (IAAsp). The polar acidic metabolite has been found to be the major metabolite in needles, shoot wood and roots, while IAAsp has been found to be the major metabolite in shoot bark. Increasing the concentration of IAA in the incubation medium resulted in an increase in the formation of a third metabolite which proved to be l‐ O ‐(indole‐3‐acetyl)‐β‐ d ‐glucose (IAGlu) and a concomitant decrease in the amount of the polar acidic metabolite. This phenomenon was prominent particularly in needles. IAGlu was isolated from needles and IAAsp was isolated from shoot bark by means of polyvinylpolypyrrolidone column chromatography and preparative thin‐layer chromatography. IAGlu was identified by comparison with authentic material by co‐chromatography in three different solvent systems and by 1 H‐nuclear magnetic resonance analysis. IAAsp was identified by comparison with authentic material by gas‐liquid chromatography and 1 H‐nuclear magnetic resonance analysis. Several aspects of formation, separation and isolation of IAA metabolites are discussed.