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Lectin‐mediated agglutination of plant protoplasts
Author(s) -
FENTON C. ARIEL LOU,
LABAVITCH JOHN M.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03323.x
Subject(s) - agglutination (biology) , lectin , agglutinin , protoplast , concanavalin a , pisum , biology , soybean agglutinin , microbiology and biotechnology , biochemistry , lactuca , botany , antibody , in vitro , immunology
The ability of concanavalin A, soybean agglutinin and lectins from Pisum sativum and Bandeiraea simplicifolia to mediate the agglutination of protoplasts prepared from Nicotiana glauca, Zea mays , and Lactuca sativa was assessed. Pea lectin failed to mediate agglutination; the other lectins agglutinated the three cell types tested. A microtiter assay was used to assess the activity of the lectins. The three active lectins had different activities against each of the protoplast types tested.