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Separation and analysis of steryl and wax esters from Dicranum elongatum
Author(s) -
KARUNEN PIRJO,
MIKOLA HEIKKI,
EKMAN RAINER
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03315.x
Subject(s) - stigmasterol , campesterol , wax , phytol , cycloartenol , chemistry , sterol , chromatography , organic chemistry , palmitic acid , hydrolysis , wax ester , fatty acid , biochemistry , cholesterol
Complete separation of the steryl and wax esters in the subarctic moss Dicranum elongatum was achieved on MgO thin‐layer plates without any notable alteration of the acyl and alkyl moieties of the esters. Gas chromatography‐mass spectrometry of the hydrolyzed fraction showed that the sterols (campesterol, stigmasterol, sitosterol, cycloartenol, 24‐methylene cycloartanol and an unidentified sterol) were primarily esterified with unsaturated fatty acids 18:2 ω 6, 18:3 ω 3 and 20:4 ω 6. In contrast, the wax alcohols (l‐octadecanol, phytol and geranylgeraniol) were mainly esterified with saturated fatty acids with 16:0, 18:0 and 20:0 as major components. No great differences were found in the fatty acid pattern of the steryl esters between different portions of the shoot. Slight differences, however, were found in the proportions of ω 3 and ω 6 fatty acids. In the wax esters a clear decrease was found in the proportions of 18:0 and 20:0 acids with increased shoot age accompanied by a slight increase in the proportions of 14:0, 20:4 ω 6 and phytenic acid.