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Nicotiana tabacum callus studies. X. ABA increases resistance to cold damage
Author(s) -
BORNMAN CHRIS H.,
JANSSON EVA
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03292.x
Subject(s) - abscisic acid , callus , pith , proline , nicotiana tabacum , explant culture , horticulture , dry weight , botany , chemistry , biology , biochemistry , in vitro , amino acid , gene
The effects of cis. trans abscisic acid on response to chilling was investigated in callused Nicotiana tabacum L. pith explants. Explants pretreated with 10 ‐4 M ABA underwent approximately 50% less cellular leakage when chilled at 2°C under short‐day conditions for 10 d than the comparable non‐treated tissue. Growth in terms of fresh and dry weights, although poor in comparison to non‐chilled (20°C, long days) treatments, was more than twice that of the non‐ABA‐treated material. On an absolute dry weight basis proline content increased on chilling from 0.7 to 3.4 mg g ‐1 in non‐ABA‐treated explants, but rose to nearly 17 mg g ‐1 in the tissue treated with ABA. Only in the case of cold‐hardened. ABA‐treated tissue could some cells survive subzero temperatures and regenerate callus again. It is suggested that at least part of the ameliorating effects of ABA result from an increase in the level of proline.