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Photosynthetic studies with mesophyll protoplasts from Notonia grandiflora, a crassulacean acid metabolism plant
Author(s) -
CHELLAPPAN K. P.,
SEENI S.,
GNANAM A.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03276.x
Subject(s) - crassulacean acid metabolism , protoplast , photosynthesis , carbon fixation , biology , bicarbonate , botany , rubisco , phosphoenolpyruvate carboxylase , metabolism , biochemistry , endocrinology
A method is described for rapid enzymatic isolation of mesophyll protoplasts and cells from the crassulacean acid metabolism (CAM) plant Notonia grandiflora DC. The mesophyll protoplasts exhibited high rates of 14 CO 2 fixation both in the light (45 μmol of CO 2 fixed mg −1 Chl h −1 ) and in the dark (20 μmol of CO 2 fixed mg −1 Chl h −1 ). The protoplasts also showed O 2 evolution (40 μmol of O 2 evolved mg −1 Chl h −1 ) without added bicarbonate. Exogenously added bicarbonate had no stimulating effect on the O 2 evolution. Analyses of early photosynthetic products in the light showed the formation of both C 3 and C 4 acids. Aspartate was found to be a predominant photosynthate.