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The effects of temperature and inhibitors of protein biosynthesis on the recovery from gas‐shock of Acer pseudoplatanus cell cultures
Author(s) -
THOIRON BERNARD,
THOIRON ARLETTE,
ESPEJO JOSÉ,
GUIEL JEANNE LE,
LÜTTGE ULRICH,
THELLIER MICHEL
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03237.x
Subject(s) - acer pseudoplatanus , puromycin , protein biosynthesis , biochemistry , shock (circulatory) , biosynthesis , cell , chemistry , biology , enzyme , medicine
The resumption of solute uptake capacity lost after gas‐shock of Acer pseudoplatanus L. cell suspension cultures is severely inhibited by low temperatures (1°C) and by inhibitors of transcription and translation of protein synthesis such as 2‐mercapto‐1 (β‐4‐pyridethyl) benzimidazole (MPB, 40 μg ml −1 ), puromycin (around 100 μg ml −1 ) and actinomycin (100 μg ml −1 ). Cells that have already attained maximum uptake capacity loose it again after less than 1 h in 40 μg ml −1 MPB. Gel‐electrophoresis of the external media of the cells shows that the release of proteins into the solution is affected by shock. The results demonstrate that proteins are involved in the mechanism of solute uptake by the cells, so that these proteins are among the factors altered during shock and recovery, and are important for the understanding of the after‐effects of shock.