Premium
Differentiation in Lilium bulbscales grown in vitro. Effects of activated charcoal, physiological age of bulbs and sucrose concentration on differentiation and scale leaf formation in vitro
Author(s) -
TAKAYAMA SHINSAKU,
MISAWA MASANARU
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03229.x
Subject(s) - activated charcoal , bulb , sucrose , sprouting , in vitro , callus , botany , chemistry , biology , horticulture , biochemistry , organic chemistry , adsorption
Organ differentiation and growth of tissue cultured bulbscales of Lilium auratum Lindl. was investigated. Benzyladenine stimulated bulb and bulbscale differentiation but inhibited root formation. Addition of activated charcoal to the medium negated the effect of BA on differentiation, while the growth of bulbs was markedly stimulated. Organ formation was also influenced by the physiological age of bulbs. From bulbscales of a 3‐week‐old bulb, a large number of bulbs and roots was formed but callus was not produced. A high sucrose concentration (90 g/1) reduced the subsequent sprouting after planting in soil. Scale leaf formation was also regulated. Sucrose (30 g/1) stimulated scale leaf formation but 90 g/1 were inhibitory.