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Analysis of newly synthesized proteins during differentiation of cultured Douglas fir cotyledons
Author(s) -
YASUDA TAKESHI,
HASEGAWA PAUL M.,
CHENG TSAIYING
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb03223.x
Subject(s) - cotyledon , explant culture , biology , polyacrylamide gel electrophoresis , callus , biochemistry , tissue culture , protein biosynthesis , botany , cytoplasm , microbiology and biotechnology , in vitro , enzyme
Addition of plant growth regulators (5 n M NAA and 5μ M BAP) to a defined basal medium stimulated adventitious bud formation of Douglas fir ( Pseudotsuga menziesii [Mirb.] Franco) cotyledon explants in culture. Cytoplasmic soluble proteins synthesized during early stages of adventitious bud formation were analyzed by electrophoresis of 3 H‐ and 14 C‐leucine labeled proteins on SDS polyacrylamide gels. Increased synthesis of low molecular weight proteins (16,000 to 20,000 daltons) was detected after 2 days in culture and reached a maximal level at day 4. When cotyledon explants cultured on bud medium for 2 days were transferred to callus medium (which suppressed adventitious bud formation), suppression of the synthesis of low molecular weight proteins was also observed, suggesting that these proteins may be associated with early stages of adventitious bud formation.