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Phenylalanine ammonia‐lyase activity in callus cultures of Pinus elliotii
Author(s) -
LAU YIULAM,
SCHELD HERBERT W.,
COWLES JOE R.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb02668.x
Subject(s) - cycloheximide , callus , phenylalanine ammonia lyase , phenylalanine , sucrose , stimulation , enzyme assay , chemistry , ammonia , botany , biochemistry , subculture (biology) , enzyme , horticulture , biology , protein biosynthesis , amino acid , endocrinology
Phenylalanine ammonia‐lyase (PAL) activity increased 8‐ to 12‐fold in pine ( Pinus elliotii Engelm.) callus tissue within 2 days after subculturing on fresh medium. Factors such as increasing the sucrose content of the media, imposing additional tissue in jury or subculturing more frequently did not cause additional stimulation of PAL activity. The rapid increase in PAL activity appeared to be due to enzyme activation, since cycloheximide did not appreciably reduce the stimulation of PAL activity. The subsequent loss of increased PAL activity with age was reduced by cycloheximide and a cool growth environment.