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Enhancement of pollen embryo formation in Datura innoxia by charcoal
Author(s) -
TYAGI AKHILESH K.,
RASHID A.,
MAHESHWARI S.C.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb02667.x
Subject(s) - stamen , datura , agar , pollen , botany , charcoal , agar plate , chemistry , biology , genetics , organic chemistry , bacteria
In recent years liquid medium has been shown to be better than agar‐gelled medium for production of haploids by anther culture. However, on addition of charcoal to agar medium the anther response in Datura innoxia Mill, increases dramatically and is better than in liquid medium. For anthers with pollen at the premitotic stage, the best result was observed with 1% charcoal in Difco agar and 1.5% in Normal agar. The effect is possibly due to adsorption of substances inhibitory to androgenesis and emanating from anthers, as well as to substances present in the nutrient medium and agar.