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Aspects of DNA, RNA and protein synthesis during somatic embryogenesis in a carrot cell suspension culture
Author(s) -
FUJIMURA TATSUHITO,
KOMAMINE ATSUSHI,
MATSUMOTO HIDEAKI
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb02659.x
Subject(s) - somatic embryogenesis , biology , daucus carota , rna , dna synthesis , protein biosynthesis , embryogenesis , dna , biochemistry , chromatin , zeatin , embryo , microbiology and biotechnology , botany , auxin , cytokinin , gene
Changes in DNA, RNA and protein content, incorporation of 3 H‐thymidine, 14 C‐uridine and 3 H‐leucine and template activity of chromatin were investigated in the early process of somatic embryogenesis in a carrot ( Daucus carota L. cv. Kurodagosun) cell suspension culture using a synchronous system. An embryogenetic culture in a medium containing 10 ‐7 M zeatin was compared with a non‐embryogenetic culture in a medium containing 10 ‐7 M zeatin and 5 x 10 ‐7 M 2,4‐D. DNA was synthesized very actively prior to and during the formation of globular embryos in the embryogenetic culture. The RNA and protein content per tube increased at an almost constant rate in both cultures, while the rate of incorporation of labelled precursors of RNA and protein rose much more prior to active DNA synthesis in the embryogenetic culture than in the non‐embryogenetic culture. Template activity of chromatin was high in the early stage of embryogenesis in the embryogenetic culture. The results obtained here showed that synthesis and turnover of RNA and protein became active prior to active DNA synthesis in the early stage of embryogenesis, and that these changes at macromolecular levels may play important roles in embryogenesis.