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Auxin biosynthesis and metabolism in isolated roots of Zea mays
Author(s) -
FELDMAN LEWIS J.
Publication year - 1980
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1980.tb02643.x
Subject(s) - auxin , apex (geometry) , zea mays , indole 3 acetic acid , biosynthesis , biology , metabolism , tryptophan , botany , root cap , chemistry , biochemistry , amino acid , enzyme , agronomy , shoot , gene , meristem
The synthesis and metabolism of indole‐3‐acetic acid (IAA) was investigated in isolated roots of corn, Zea mays L. Roots were cultured aseptically in media supplemented with either 14 C‐tryptophan or 14 C‐IAA. Exogenously supplied IAA is rapidly and completely metabolized by root tissues. The main site in the root for the synthesis of IAA is in the apex. Removal of either the root cap or the quiescent center, or the root cap and the quiescent center from the apex has no effect on the IAA‐synthesizing ability of the apex. Subdividing the terminal 2.1 cm of the root into various segments and culturing them separately stimulates IAA synthesis in these isolated root tissues. Roots in culture maintain relatively constant IAA levels, reflecting the precise controls of the level of this hormone.