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Cultivar Differences in Starch Content and Protoplast Yields from Root Cortical Ex plants of Pisum salivum
Author(s) -
JEAN CROWDER ANTOINETTE,
LANDGREN CRAIG R.,
ROCKWOOD LARRY L.
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06536.x
Subject(s) - protoplast , sativum , pisum , cultivar , starch , biology , seedling , botany , germination , horticulture , food science
Protoplasts were isolated from root cortical explants of thirteen cultivars of Pisum sativum . The roots were tested for starch in the cortical tissues. There was a considerable range in the average protoplast yields and the coefficient of variation of those yields from the thirteen cultivars. Cultivars with a higher incidence of starchy roots had lower average explam protoplast yields and greater variation in their protoplast yields. Significantly higher protoplast yields were observed from explants with no starch or few starchy cells. Increasing the germination time of the peas increased the starch content in the seedling roots and decreased the protoplast yields.