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Genetic Analysis in Wood‐Decaying Fungi I. Genetic Variation and Evidence for Allopatric Speciation in Pleurotus ostreatus using Phenoloxidase Zymograms and Morphological Criteria
Author(s) -
PRILLINGER HANSJÖRG,
MOLITORIS H. PETER
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06533.x
Subject(s) - allopatric speciation , laccase , biology , pleurotus ostreatus , genetic variation , botany , microevolution , genetic variability , gene , enzyme , genetics , population , biochemistry , genotype , mushroom , demography , sociology
Using electropherograms (zymograms) of the phenoloxidase laccase and characteristics of mycelial growth and fruit body production, a distinct morphological and biochemical differentiation of two geographically isolated (allopatric) populations of the wood‐rotting basidiomycete Pleurotus ostreatus became evident. No limitation in their outbreeding ability was observed, however. A specific secretory mechanism for an extracellular laccase, genetically different in the two geographical races, could be detected. An approximately 1: 1 segregation of this laccase band in the F 1 generation indicates that specific secretion of this enzyme is controlled by one gene only, Different degrees of genetic variation as shown by differences in the respective laccase spectra were found in the two geographical races. Only one enzyme band out of nine multiple laccases was found to be specific for fruit bodies. The value of zymograms for chemotaxonomic purposes, for the understanding of microevolution and for determination of genetic variation in fungi is critically discussed.