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Tissue and Cell Culture of ‘Passe Crassane’ Pears: Amylase Pattern of Cultured Tissues Compared with Whole Fruit
Author(s) -
PECH JEANCLAUDE,
LATCHÉ ALAIN,
FALLOT JEAN
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06532.x
Subject(s) - amylase , biology , in vitro , explant culture , tissue culture , biochemistry , polyacrylamide gel electrophoresis , cell culture , enzyme , botany , genetics
Calluses and cell suspension cultures were initiated from young and fully developed fruits and from stem and petiole tissues of ‘Passe Crassane’ pears. On a dry weight basis, proliferation is significantly higher in tissues from full‐grown fruits and petioles. Cullus tissues grown in vitro have a protein content up to 25 times higher than the initial quiescent organ. Three amylase fractions (2 β amylases E, and E 2 : 1 α amylase E 3 ) were isolated by gel filtration on Sephadcx G‐100 and isoenzymes revealed in these fractions after polyacrylamide gel electrophoresis. Comparison of amylase activity in actively growing cells and in auxin‐starved cell suspensions shows that β‐amylase activity is mainly a function of cell growth, while α‐amylase activity is more related to the age of the cells. The presence of two isoenzymes in the β‐amylase E 2 fraction was found to be highly characteristic of young fruits. The same polymorphism was found in all the strain of tissues thus indicating that tissues grown in vitro retain or revert to a juvenile biochemistry. The growth hormone. 2,4‐dichlorophenoxyacetic acid, used to support cell division, is demonstrated to be responsible for the reversion of the β‐amylase polymorphism from a mature to a juvenile phenotype in mature fruit explants incubated in vitro . The interest of fruit tissue and cell culture for the study of fruit physiology is questioned and discussed.