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Self inhibition of the Agaricus bisporus Spore by CO2 and/or γ‐Glulaminyl‐4‐hydroxybenzene and γ‐Gtutaminyl‐3,4‐benzoquinone: A Biochemical Analysis
Author(s) -
RAST DORA,
STÜYSSI HANS,
ZOBRIST PETER
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06527.x
Subject(s) - agaricus bisporus , spore , spore germination , agaricus , chemistry , in vivo , biochemistry , mushroom , biology , botany , food science , microbiology and biotechnology
Chemical and enzymological analyses have been carried out to study the mode of action of CO 2 , in inhibiting germination in Agaricus bisporus and to test the suggestion (Vogel and Weaver, 1972. Exp. Cell Res. 75: 95, Vogel et at, 1974. Am. J. Pathol. 76: 165) that γ‐glutaminyl‐4‐hydroxybenzene (GHB) and/or γ‐glutaminyl‐3,4‐benzoquinone (GBQ) would inhibit the succinate dehydrogenase (SDH) in the mushroom spore and thus represent the causal agent(s) of its dormancy. Evidence is presented that the impairment of SDH is brought about by Ihe CO, fixation product oxaloacelate which is formed in the pyruvate carboxylase reaction, and a mechanism is proposed for the release of the SDH inhibition when activating the spores. These are almost devoid of GHB, whereas fruit bodies of all developmental stages contain considerable amounts of the phenol. GBQ is absent in the spore, and its occurrence in mushroom fruit body extracts appears to be an artifact of extraction. Apart from these analytical results, theoretical reasons are given that vouch against the involvement of the phenolics in the inhibition of SDH in vivo .