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Chlorophyll Composition in an Isolated Chlorophyll a ‐Protein Complex of Photosystem I
Author(s) -
ANDERSSON LARS,
EGNÉUS HANS
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06503.x
Subject(s) - pheophytin , p700 , photosystem i , photosystem ii , chlorophyll , light harvesting complexes of green plants , chemistry , chlorophyll a , photosynthesis , photosynthetic reaction centre , column chromatography , chromatography , biochemistry , organic chemistry
A P700‐chlorophyll a ‐protein complex, solubilized by the detergent Triton X‐100, has been isolated by hydroxyl apatite column chromatography. The chlorophyll composition was determined by thin‐layer chromatography and spectrofluorimetric analysis. This photosystem I reaction centre complex, prepared at pH 7, contained pheophytin a and P700 in a ratio of 2/1, high enough to account for a composition similar to that in the reaction centre of photosynthetic bacteria. Prepared at pH 9, the same ratio was 0.2/1, which excludes pheophytin a from having the same function as that of bacterio‐pheophytin in the photosynthetic bacteria.