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Isotachophoretic Analysis of Ions in Guard Cells of Vicia faba
Author(s) -
SHIMADA KEIZO,
OGAWA TERUO,
SHIBATA KAZUO
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06303.x
Subject(s) - guard cell , vicia faba , malic acid , chemistry , phosphoric acid , potassium , glyceric acid , citric acid , sodium , ion , botany , horticulture , chromatography , biochemistry , nuclear chemistry , biology , organic chemistry , catalysis
Isotachophoretic analysis of ions was performed on guard cells of Vicia faba cv. Ryosai Issun with either open or closed stomata. In guard cells of open stomata, K + and malic acid concentrations were 5–7 and 5–10 times higher, respectively, than in guard cells of closed stomata. The content of citric acid ( plus isocitric acid) also increased during stomatal opening, but the increment was smaller than that of malic acid. Sodium ions, phosphoric and glyceric acids were present in low concentrations but did not increase during the opening. Other cations and anions could not be measured because of low concentrations. Malic acid provided 68–79% of the counter anions for the potassium taken up by guard cells during stomatal opening.