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Effect of Purine Analogs and Their Interactions with Benzyladenine on Bud Burst and Shoot Growth in Apple Bud Explants
Author(s) -
SINGHA SUMAN,
POWELL LOYD E.
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb06302.x
Subject(s) - explant culture , purine , axillary bud , biology , shoot , horticulture , malus , pyrimidine , botany , biochemistry , in vitro , enzyme
The purine analogs 8‐azaadenine, 8‐azaguanine, 4‐aminopyrazolo [3,4‐d] pyrimidine, 2‐fluoroadenine and 6‐methylpurine inhibited bud burst in non‐dormant vegetative axillary bud explants obtained from apple ( Malus domestica Borkh.) seedlings. The reversal of inhibition was attempted by incorporating increasing concentrations of benzyladenine (BA) in the culture medium. The inhibition caused by 8‐azaadenine and 8‐azaguanine was reduced especially when BA was added to the media at equimolar ratios along with the inhibitors. The inhibition due to 4‐aminopyrazolo [3,4‐d] pyridine was reduced only with equimolar BA, BA was ineffective or only minimally effective in reducing inhibition due to 2‐fluoroadenine and 6‐methypurine. Short pretreatment with 8‐azaadenine and 8‐azaguinine failed to cause any inhibition when explants were subsequently transferred to BA ‐containing medium. The effectiveness of 2‐fluoroadenine and 6‐methylpurine was maintained or only slightly reduced, indicating their long‐term inhibitory action.