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Induction of Nitrate Reductase and Peroxidase Activity in the Seedlings of Normal and High Lysine Maize
Author(s) -
SRIVASTAVA H. S.,
ASTHANA J. S.,
JAIN ABHA
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb03201.x
Subject(s) - endosperm , nitrate reductase , peroxidase , nitrate , shoot , enzyme assay , cultivar , enzyme , lysine , botany , chemistry , biology , horticulture , agronomy , biochemistry , amino acid , organic chemistry
Steady state levels of in vivo nitrate reductase activity in the endosperm, scutella, roots and shoots of maize seedlings were higher in normal maize than those in high lysine maize. Activity of peroxidase in the roots, however, was higher in the high lysine cultivar. The nitrate reductase activity increased with the supply of nitrate in all parts of the seedlings of both cultivars, although the rates of increment in the endosperm were lower than those in scutella, roots and shoots. In relation to substrate concentration, a saturation was achieved at 5 to 10 m M of nitrate except in the endosperm, where activity increased slowly up to 100 m M at least. Final levels of enzyme activity were significantly higher in the scutella of normal than in that of high lysine seedlings. In vitro enzyme activity in the roots also increased with the supply of nitrate in both cultivars, reaching maximum at 5 to 10 m M nitrate.