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Oxygen Consumption Stimulated by δ‐Aminolevulinic Acid in Darkness and during Irradiation of Dark Grown Wheat Leaves
Author(s) -
CARLSSON ROLF,
SUNDQVIST CHRISTER
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb03199.x
Subject(s) - darkness , protochlorophyllide , oxygen , chemistry , potassium cyanide , respiration , biochemistry , cyanide , potassium , biology , botany , enzyme , inorganic chemistry , biosynthesis , organic chemistry
Dark grown wheat leaves ( Triticum aestivum L. cv. Starke II Weibull), treated with δ‐aminolevulinic acid in darkness, showed an increased oxygen uptake as measured by a Warburg method. The production of CO 2 was also increased in darkness, giving an RQ ≅ 1. The increased respiration was dependent on the treatment time as well as on the concentration of the δ‐aminolevulinic acid. Potassium cyanide suppressed both the normal and the increased respiration. The treatment with δ‐aminolevulinic acid caused accumulation of high amounts of protochlorophyllide. Levulinic acid suppressed the increased oxygen uptake as well as the protochlorophyllide accumulation in δ‐aminolevulinic acid treated leaves. Irradiation rapidly decreased the protochlorophyllide content with a simultaneous increase in oxygen uptake over the dark value. The peak value of the increase in oxygen uptake was reached after about 5 min. The light induced oxygen uptake was dependent on the amount of PChlide present at the onset of irradiation. Also the CO 2 production was increased during the first minutes of irradiation but soon fell under the buffer control value. Neither potassium cyanide nor heat denaturation affected the oxygen uptake in light in contrast to the effect on the CO 2 production, which was blocked by heat denaturation. The increased oxygen uptake in light initially seems to be a purely photochemical process leading to a release of CO 2 , which release is probably an enzymatic process induced by the photo‐oxidative decomposition of pigment.