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Alterations in Endogenous Levels of Gibberellin‐like Substances during Germination of Phaseolus vulgaris Seeds
Author(s) -
SCHIRACHSZMIGIEL LINDA
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb03185.x
Subject(s) - phaseolus , gibberellin , germination , ethyl acetate , bioassay , serial dilution , butanol , chemistry , endogeny , botany , chromatography , horticulture , biology , biochemistry , ethanol , medicine , alternative medicine , pathology , genetics
The endogenous gibberellin‐like substances were determined in mature dry and germinating bean seeds, Phaseolus vulgaris L. cv. Alabaster. Methanol extracts were partitioned against ethyl acetate and butanol at neutral and acid pH. Each phase was individually chromatographed on a silica gel column. The gibberellin activity was measured with the Tan‐ginbozu dwarf rice micro‐drop bioassay. Each extract was tested in two dilutions. Extracts from dry seeds showed the highest gibberellin activity, largely attributable to ethyl acetate‐soluble substances. The activity was considerably reduced in extracts from seeds imbibed for 1 day. Gibberellin‐like substances soluble in butanol appeared in extracts from seeds soaked for 1 or more days.