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Ethylene Production by Tobacco ( Nicotiana tabacum ) Callus
Author(s) -
HUXTER TERRY J.,
REID DAVID M.,
THORPE TREVOR A.
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb02635.x
Subject(s) - ethylene , callus , nicotiana tabacum , chemistry , botany , growth inhibition , horticulture , biology , biochemistry , cell growth , catalysis , gene
Tobacco callus cultures grown on defined agar‐solidified media produced ethylene in differing amounts, which were related to cultural treatment and age of the callus. There was a close correlation between the rate of ethylene production and growth. In darkness, maximal rates occurred in the third week of growth with ethylene production in the range of 750 nl (callus piece) −1 d −1 (fr. wt. = 1.5 g), and in the light, maximal rates occurred in the first week of growth, 200 nl (callus piece) −1 d −1 (fr. wt. = 200 mg). Growth was also correlated with ethylene production when the latter was altered by exposure of the callus to inhibitors of ethylene synthesis, L‐canaline, benzyl isothiocyanate, and 3,5‐diiodo‐4‐hydroxy‐benzoic acid. No correlation was found following treatment with AgNO 3 , a presumptive inhibitor of ethylene action. The inhibition of growth and ethylene production by L‐canaline was partially reversed by gassing the cultures with ethylene (1 μl/1). A mercuric perchlorate sink had no significant effect on growth. A possible relationship between ethylene evolution and growth is discussed.