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Action Spectra for in vivo and in vitro Conversions of Phycochrome b, a Reversibly Photochromic Pigment in a Blue‐Green Alga, and Its Separation from Other Pigments
Author(s) -
BJÖRN G. S.
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb02622.x
Subject(s) - pigment , in vivo , photochromism , chemistry , in vitro , action spectrum , stereochemistry , biophysics , photochemistry , biochemistry , organic chemistry , biology , microbiology and biotechnology
Phycochrome b, one of the reversibly photochromic pigments found in Tolypothrix distorta seems to exist in only two forms: P b 500 and P b 570 . The pigment has been spectroscopically demonstrated in vivo . It has also been separated from other pigments. Two different methods for separation have been used: isoelectric focusing and gel filtration. Preparations of purified phycobilisomes contain phycochrome b. The in vivo and in vitro absorption difference spectra were determined as well as action spectra for the conversions in vitro and in vivo of P b 500 to P b 570 and vice versa . Transformation kinetics of phycochrome b show that the conversions in both directions are initially first‐order reactions.