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Isolation and Mobilization of Storage Proteins from Apple Shoot Bark
Author(s) -
O'KENNEDY BRENDAN T.,
TITUS J. S.
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb02606.x
Subject(s) - storage protein , bark (sound) , chemistry , polyacrylamide gel electrophoresis , amino acid , chromatography , cellulose , polyacrylamide , electrophoresis , column chromatography , biochemistry , biology , enzyme , ecology , gene , polymer chemistry
Separation of the bark storage protein into three groups was achieved with minimal contamination from interfering cbromophores, using diethylaminoethyl cellulose column chromatography. Two of these groups were shown 10 contain neutral sugars in close association with the proteins. All three groups disappeared rapidly after the onset of growth. Polyacrylamide gel electrophoresis of the storage proteins showed the presence of eight prominent proteins. Ammo acid analysis of hydrolyzates of the proteins indicated that a large percentage of the protein nitrogen was in the form of basic amino acids.