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Influence of Growth Regulators on Root and Shoot Initiation from Flax Meristem‐Tips and Hypocotyls in vitro
Author(s) -
LANE W. DAVID
Publication year - 1979
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1979.tb01698.x
Subject(s) - hypocotyl , meristem , gibberellic acid , shoot , biology , botany , germination
Hypocotyl sections and isolated meristem‐tips from 7‐ to 10‐day‐old seedlings of flax were cultured on a Murashige and Skoog (1962) medium. The optimum concentrations of naphthaleneacetic acid (NAA) and benzyladenine (BA) were determined for shoot and root initiation from hypocotyls. Meristem‐tip cultures differentiated shoots and roots in response to these concentrations. The frequency and yield of shoots, promoted by BA, decreased if NAA or gibberellic acid (GA 3 ) was included in the medium. At high NAA concentration GA 3 and BA inhibited root initiation. Morphological development of cultures differed depending on the growth regulators in the medium.