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Induction of Adventitious Buds on Embryos of Norway Spruce Grown in vitro
Author(s) -
ARNOLD SARA VON,
ERIKSSON TAGE
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb08633.x
Subject(s) - primordium , picea abies , embryo , biology , shoot , botany , in vitro , microbiology and biotechnology , biochemistry , gene
Adventitious buds were induced when isolated embryos of Norway spruce ( Picea abies L.) were cultured on a defined medium containing 2iP. Anatomically different bud primordia were formed at high and low cytoldnin concentrations. The highest percentage of embryos forming bud primordia was obtained after 4–5 weeks on 2iP medium. Buds developed after transfer of the induced embryos to medium without growth regulators. Many of the buds developed into elongated shoots. No root primordia were observed in any of the induced embryos.