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Factors Affecting Ethanol Induced Luminescence in Dark‐Treated Chloroplasts
Author(s) -
SIGFRIDSSON BJÖRN
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb08628.x
Subject(s) - ethanol , chloroplast , chemistry , photochemistry , luminescence , chlorophyll , ferricyanide , singlet oxygen , acetone , sodium dodecyl sulfate , inorganic chemistry , oxygen , chromatography , biochemistry , materials science , organic chemistry , optoelectronics , gene
Dark‐treated chloroplasts emit light when treated with a high ethanol concentration. The ethanol treatment causes chlorophyll solvation. The light is red and emanates from a singlet excited molecule, probably a chlorophyll peroxide. It is quenched by acetone, sodium dodecyl sulfate treatment of the chloroplasts before the addition of ethanol, boiling, reducing substances and low pH. It is enhanced by ferricyanide and high pH. This is interpreted as a requirement for an organized structure and for an energy transfer system for light emission to occur.