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Seasonal Effects on Chlorophyll‐Protein Complexes Isolated from P‐inus silvestris
Author(s) -
ÖQUIST GUNNAR,
MÅRTENSSON OLLE,
MARTIN BJÖRN,
MALMBERG GUNILLA
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb08616.x
Subject(s) - p700 , chlorophyll , chlorophyll a , photosystem i , chloroplast , photobleaching , light harvesting complexes of green plants , chemistry , photosystem ii , photosystem , botany , photosynthesis , biology , fluorescence , biochemistry , physics , quantum mechanics , gene
The seasonal changes in the relative distribution of P700 chlorophyll‐protein complex a 1 and light harvesting chlorophyll‐protein complex a/b were studied in a natural stand of Pinus silvestris . Similar measurements were made after artificial photobleaching of chlorophyll in pine seedlings or in isolated pine chloroplasts. The chlorophyll‐protein complexes were solubilized by sodium dodecyl sulphate and separated by polyacrylamide gel electrophoresis. When autumn and winter destruction of chlorophyll occurs, the chlorophyll a antenna associated with P700 in photosystem 1 (P700‐CPa 1 ) is relatively more affected than the light harvesting complex, which lacks a reaction centre. These results are further supported by low‐temperature fluorescence emission properties of isolated chloroplasts presented in this work and elsewhere. The destruction of chlorophyll in stressing autumn and winter climates is most probably caused by photosensitized oxidation of chlorophyll.