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Adenosine 3′:5′‐Cyclic Monophosphate in Normal and Habituated Helianthus Callus
Author(s) -
TRUELSEN T. A.,
WYNDAELE RITA
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb04090.x
Subject(s) - callus , adenosine , protein kinase a , phosphodiesterase , helianthus annuus , biochemistry , auxin , biology , chemistry , kinase , enzyme , botany , horticulture , sunflower , gene
A compound with properties identical to adenosine 3’:5′‐cyclic monophosphate (cAMP) has been isolated from callus of Helianthus annuus . The compound accompanied cAMP during an extensive purification procedure and co‐chromatographed with cAMP in three solvent systems. The compound stimulated cAMP‐dependent protein kinase and was active in a cAMP protein‐binding assay. Its activity was abolished by cAMP phosphodiesterase. It is concluded that the compound is cAMP. The variation in cAMP content in the callus during its development was determined. In 1974 when the stock callus was grown on auxin‐containing medium the content was c . 2 pmol cAMP/g when the cells were vigorously dividing and c . 125 pmol cAMP/g two weeks after the transfer. In 1976 the callus had attained hormone autotrophy. Its cAMP content was generally low during the whole developmental cycle. The results might indicate that cAMP played a role in the development of the normal callus of 1974, while it has no regulatory function in the tumour‐like tissues of 1976. The results are discussed on the basis of recent literature.