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Half‐life of tRNA Containing N 6 (Δ 2 ‐isopentenyl)adenosine in Lactobacillus acidophilus ATCC 4963
Author(s) -
HELBACH MAX,
LEINEWEBER MICHAEL,
KLÄMBT DIETER
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01628.x
Subject(s) - mevalonic acid , lactobacillus acidophilus , transfer rna , methionine , stereochemistry , adenosine , cytokinin , biochemistry , chemistry , amino acid , biosynthesis , biology , rna , bacteria , auxin , enzyme , probiotic , gene , genetics
tRNA containing N 6 ‐(Δ 2 ‐isopentenyl)adenosine may be precursors for the plant hormone cytokinin. To discriminate between tRNA containing and not containing cytokinin nucleotides, double labelling experiments were made by the use of [2 14 C]‐mevalonic acid and [ 3 H‐methyl]‐methionine. At a generation cycle of 2 h for Lactobacillus acidophilus ATCC 4963, the half‐lives of tRNA labelled with [ 3 H‐methyl]‐methionine and [2‐ 14 C]‐mevalonic acid are similar, namely 3 h. Isopentenylation of tRNA could be measured to be maximally 1:10.