Premium
The Metabolism of the Herbicide Diphenamid ( N‐N ‐dimethyl‐2,2‐diphenyl‐acetamide) in Cell Suspensions of Soybean ( Glycine max )
Author(s) -
DAVIS D. G.,
HODGSON R. H.,
DUSBABEK K. E.,
HOFFER B. L.
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01619.x
Subject(s) - glycine , acetone , metabolism , chromatography , chemistry , cell culture , biochemistry , biology , amino acid , genetics
The fate of the herbicide diphenamid was determined in cell suspensions of soybean [ Glycine max (L.) Merr. ‘Wilkin’] at different stages of cell growth: early log phase (3 to 7 d), log phase (7 to 14 d), and stationary phase (14 to 18 d). [Carbonyl‐ 14 C]‐diphenamid was added to the suspensions as an acetone solution. Neither diphenamid (2 to 3 μM ) nor acetone (0.5% v/v) was phytotoxic. The 14 C‐labeled products were identified tentatively by thin layer chromatographic comparison with reference compounds. The major metabolic products formed were N ‐hydroxymethyl‐ N ‐methyl‐2,2‐diphenylacetamide, N ‐methyl‐2,2‐diphenylacetamide, 2,2‐diphenylacetamide, and two polar metabolites (0.9 to 25% of the applied 14 C activity) that appeared to be glucose conjugates; one an acidic glucoside. All metabolites were found in both the cell extract and the culture medium, except for the acidic glucoside, which was recovered in small amounts only from the cell extracts. These products were the same as those recovered from intact plants. Similar results were obtained from cell suspensions of different ages. The rate of metabolism by log phase cells was slightly less than the rate for either young or old cells. The results indicated that soybean cell suspensions can be used to obtain reliable information on the fate of agricultural chemicals in soybeans.