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Changes in Gene Expression from Diploid to Autotetraploid Status of Lycopersicon esculentum
Author(s) -
ALBUZIO A.,
SPETTOLI P.,
CACCO G.
Publication year - 1978
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1978.tb01617.x
Subject(s) - phosphoenolpyruvate carboxylase , photorespiration , lycopersicon , biochemistry , pyruvate carboxylase , malate dehydrogenase , biology , ploidy , photosynthesis , glutamate dehydrogenase , nitrate reductase , glutamine synthetase , enzyme , carbon fixation , ribulose , botany , gene , amino acid , glutamate receptor , glutamine , receptor
The activity levels of enzymes involved in photosynthesis, respiration and nitrogen metabolism were evaluated in a diploid and in the derived autotetraploid Lycopersicon esculentum . The autotetraploid exhibited, compared to the diploid, higher protein and reduced dry matter content; reduced rate of protein synthesis; enhanced activity of malate dehydrogenase, acid invertase, glutamate dehydrogenase, and nitrate reductase; decreased peroxidase activity; unchanged polymorphism of the above‐listed enzymes as revealed by electrofocusing, namely reduced CO 2 fixation caused by lower ribulose‐1‐5‐diphosphate carboxylase and even lower phosphoenolpyruvate carboxylase activities in accordance with a smaller contribution of the C 4 carboxylating cycle; lower activities of the two “key” enzymes of photorespiration ribulose‐1‐5‐diphosphate oxygenase and glycolate oxydase. The results suggest that the gene expression of the double gene dosage is enhanced in some cases and depressed in others, resulting in more unfavourable balance of the metabolic settlement.